Structural Analysis in Situ by Super-resolution Imaging and vEM in Disease and Toxic States
We have developed a strategy for building nanometer-scale molecular maps of organelle structure and organization by combining super resolution microscopy (3DSIM and STORM) and volumetric Electron Microscopy techniques such as FIB-SEM. This strategy allowed us to resolve several structures associated with cilia, centrosomes and flagella. We are now taking a comprehensive approach to study cells organisation and content in tissues to reveal mechanisms linked to toxic responses to pollutants and better understand disease states.
Nguyen Q et al. Developmental Cell 2020
Fishman et al. Nature Communications 2018
Sydor A et al. Trends in Cell Biology 2015
Mennella V et al. Methods in Cell Biology 2014
Mennella V Encyclopedia in Cell Biology 2014, 2022
Mennella V et al. Nature Cell Biology 2012
Development of Imaging Toolbox for Diagnosis of Motile Ciliopathies
We have recently extended our imaging approach to develop methods that have clinical impact by solving difficult cases of molecular diagnosis intractable by current methods such EM and DNA sequencing
Liu et al. Science Translational Medicine, 2020
Mennella et al. Encyclopedia of Respiratory Medicine, Elsevier, 2020 accepted
Cilia Signalling in Disease and Toxic States
The lab is interested in understanding the mechanisms by which cilia communicate with the environment to respond to infections and toxic insults. We have recently discovered with our colleagues at U. of Southampton that airway cells express ACE-2, the receptor of the SARS-CoV2 virus on the surface of motile cilia. We are now investigating its function, targeting and binding partners with support from UKRI-BBSRC.
Blume et al. Nature Genetics 2022
Sydor A et al. eLife 2018
Cilia and Centrosomes in Disease and Toxic States
Blume et al. Nature Genetics 2022
Sydor A et al. eLife 2018